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hf buffer  (New England Biolabs)


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    Structured Review

    New England Biolabs hf buffer
    Hf Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1656 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phusion+hf+buffer/Phusion+HF+Buffer+Pack/pmc13680278-269-12-14
    Average 96 stars, based on 1656 article reviews
    hf buffer - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    other:

    Article Title: Longitudinal Characterization of Gingival Microbiota Following Periodontally Accelerated Osteogenic Orthodontics Surgery in Skeletal Class III Orthognathic Patients.
    Article Snippet: BI PD KEG (mm) (mm) w 0 2w 4w 0 2w 4w 0 2w 4w 2 1 1 3 3.5 3 4.0 4.5 4.7 1 1 1 2 2 2 5.3 5.7 5.9 1 2 1 2 3 2 4.5 5.1 5.3 1 1 1 2 2 3 6.0 6.4 6.5 1 2 1 2 2 2 4.8 5.3 5.7 1 2 2 2 3 2 5.4 5.8 6.0 inized gingiva width (mm); PD, probing depth (mm); PLI, plaque index; Adaptors were then ligated to the DNA fragments by mixing 5 ml of digested DNA with 10 ml of a ligation master mix containing 0.2 mM of each adaptor and 800 U of T4 DNA ligase (NEB).

    Article Title: Trisomy 21 alters ciliary localization of Sonic Hedgehog signaling proteins
    Article Snippet: cDNA was synthesized from isolated RNA using SuperScript TM IV First-Strand Synthesis System (18091050; Invitrogen) according to manufacturer’s protocol.

    Polymerase Chain Reaction:

    Article Title: High-throughput detection of microeukaryotic parasites in insects using Nanopore sequencing
    Article Snippet: .. Each 20 μl PCR reaction contained 2 μl of DNA (~20 ng), 1 μM primers, 200 μM dNTPs, 1× Phusion HF Buffer and 0.02 U Phusion DNA polymerase (NEB). ..

    Article Title: High-throughput detection of microeukaryotic parasites in insects using Nanopore sequencing.
    Article Snippet: .. Each 20 μl PCR reaction contained 2 μl of DNA (~20 ng), 1 μM primers, 200 μM dNTPs, 1× Phusion HF Buffer and 0.02 U Phusion DNA polymerase (NEB). ..

    Article Title: Intratumoral immune-microbial crosstalk shaped by tumor cell-derived extracellular vesicles encapsulating berberine boosts lung cancer immunotherapy
    Article Snippet: .. Each 20 μL PCR reaction included 25 ng of gel-extracted PCR product, 0.2 μmol/L of each primer, 0.3 mmol/L dNTPs, 1 × Phusion HF buffer, and 0.4 units of Phusion high-fidelity DNA polymerase (NEB). .. After purification using the QIAquick PCR Purification Kit (Qiagen), the libraries were sequenced on the Illumina NovaSeq PE150 platform.

    Article Title: Longitudinal Characterization of Gingival Microbiota Following Periodontally Accelerated Osteogenic Orthodontics Surgery in Skeletal Class III Orthognathic Patients
    Article Snippet: .. Each 20 μl PCR reaction contained 25 ng of gel-extracted product, 0.2 μM of each primer, 0.3 mM dNTP, 1 × Phusion HF buffer, and 0.4 U Phusion high-fidelity DNA polymerase (NEB). .. PCR products were purified with the QIAquick PCR purification kit (Qiagen) and sequenced on the Illumina Nova PE150 platform.

    Article Title: Error-free and efficient prime editing in absence of maternal Polθ
    Article Snippet: .. Each PCR well contained: 5.13 μL dH2O, 1.8 μL 5× Phusion HF buffer (New England Biolabs), 0.3 μL DMSO, 0.18 μL dNTP (10 mM), 0.45 μL forward primer (10 μM), 0.45 μL reverse primer (10 μM), 0.09 μL Phusion High-Fidelity DNA Polymerase (New England Biolabs), 1.0 μL genomic DNA; for a total of 9.4 μL. ..

    Amplification:

    Article Title: Universal Baseline for in vitro Selection of Genetically Encoded Libraries
    Article Snippet: .. The method was adapted from our previous developed protocol. ( , ) DNA template (phage solution) was amplified in Phusion® HF buffer with Phusion® High-Fidelity DNA Polymerase (NEB, #M0530S). ..



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